MorphoLibJ provides two plugins under the Plugins › MorphoLibJ › Binary Images… menu to apply this strategy on 2D and 3D images: Distance Transform Watershedĭistance Transform Watershed needs one 2D 8-bit binary image to run. From left to right: sample image of touching DAPI stained cell nuclei from a confocal laser scanning microscope, binary mask calculated after filtering and thresholding input image, inverse of the distance transform applied to the binary mask (Chamfer distance map using normalized Chessknight weights and 32-bit output) and resulting labeled image after applying watershed to the inverse distance image using the binary mask (dynamic of 1 and 4-connectivity). See the GNU General Public License for more details.Basics of the Distance Transform Watershed algorithm. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. This program is free software you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation ( ). Bertrand's Topological Watershed site, with papers, lecture slides and source code.
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